Botley Road was turnpiked in 1767 as part of the improvements of the road from Oxford to Eynsham, and a turnpike gate was set up near Osney Bridge around this time.

Later in the eighteenth century, when a new coach road was built up Cumnor Hill, the Botley Road also became the first stage of the turnpike from Oxford to Faringdon.

The original tollhouse near Osney Bridge had to be demolished to make room for the railway, and a new tollhouse was built in 1850: this later became the Old Gatehouse pub (now the White House). The turnpike gate was moved to the west of Binsey Lane in 1868, and to the foot of Cumnor Hill in 1877.

The Oxford to Faringdon Road was deturnpiked in 1880.